Publications

Publications

2018

Dai J, Qiu W, Wang N, Nakanishi H, Zuo Y
Comparative transcriptomic analysis of the roots of intercropped peanut and maize reveals novel insights into peanut iron nutrition.
Plant Physiol Biochem 127: 516–524.

2017

Bashir K, Nozoye T, Nagasaka S, Rasheed S, Miyauchi N, Seki M, Nakanishi H, Nishizawa NK
Paralogs and mutants show that one DMA synthase functions in iron homeostasis in rice.
J Exp Bot 68: 1785–1795.

Masuda H, Shimochi E, Hamada T, Senoura T, Kobayashi T, Aung MS, Ishimaru Y, Ogo Y, Nakanishi H, Nishizawa NK
A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil.
PLOS ONE 12: e0173441

Nozoye T, Aung MS, Masuda H, Nakanishi H, Nishizawa NK
Bioenergy grass [Erianthus ravennae (L.) Beauv.] secretes two members of mugineic acid family phytosiderophores which involved in their tolerance to Fe deficiency.
Soil Sci Plant Nutr 63: 543–552.

Nozoye T, Otani M, Senoura T, Nakanishi H, Nishizawa NK
Overexpression of barley nicotianamine synthase 1 confers tolerance in the sweet potato to iron deficiency in calcareous soil.
Plant Soil 418: 75–88.

Senoura T, Sakashita E, Kobayashi T, Takahashi M, Aung MS, Masuda H, Nakanishi H, Nishizawa NK
The iron-chelate transporter OsYSL9 plays a role in iron distribution in developing rice grains. Plant Mol Biol 95: 375–387.

2016

Aung MS, Masuda H, Kobayashi T, Kakei Y, Tun YT, Nakanishi H, Yamakawa T, Nishizawa NK
Identification of mineral-rich rice varieties in Myanmar.
Myanmar Agricultural Research Journal, September 14–29.

Kobayashi T, Itai RN, Senoura T, Oikawa T, Ishimaru Y, Ueda M, Nakanishi H, Nishizawa NK
Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.
Plant Mol Biol 91: 533–547.

Shin Y, Takahashi R, Nakanishi H, Yamakawa T
Sweet potato expressing the rice Zn transporter OsZIP4 exhibits high Zn content in the tuber.
Plant Biotechnol 33: 99–104.

Suzuki M, Nozoye T, Nagasaka S, Nakanishi H, Nishizawa NK, Mori S
The detection of endogenous 2’-deoxymugineic acid in olives (Olea europaea L.) indicates the biosynthesis of mugineic acid family phytosiderophores in non-graminaceous plants.
Soil Sci Plant Nutr 62: 481–488.

Trijatmiko KR, Dueñas C, Tsakirpaloglou N, Torrizo L, Arines FM, Adeva C, Balindong J, Oliva N, Sapasap MV, Borrero J, Nakanishi H, et al
Biofortified indica rice attains iron and zinc nutrition dietary targets in the field.
Sci Rep 6: 19792.

Vigani G, Bashir K, Ishimaru Y, Lehmann M, Casiraghi FM, Nakanishi H, Seki M, Geigenberger P, Zocchi G, Nishizawa NK
Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants.
J Exp Bot 67: 1357–1368.

2015

Akhtar S, Kakei Y, Bashir K, Shahzad A, Yamakawa T, Arshad M, UL-Hassan F, Nakansihi H, Nishizawa NK
Characterizing the expression of genes involved in iron transport in Pakistani peanut varieties under iron deficiency stress.
Turkish J Bot 39: 996–1007.

Bashir K, Ishimaru Y, Itai RN, Senoura T, Takahashi M, An G, Oikawa T, Ueda M, Sato A, Uozumi N, Nakanishi H, Nishizawa NK
Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice.
Plant Mol Biol 88: 165–176.

Ito S, Nozoye T, Sasaki E, Imai M, Shiwa Y, Shibata-Hatta M, Ishige T, Fukui K, Ito K, Nakanishi H, Nishizawa NK, Yajima S, Asami T
Strigolactone regulates anthocyanin accumulation, acid phosphatases production and plant growth under low phosphate condition in Arabidopsis.
PLoS One 10: e0119724.

Nakanishi H, Mori A, Takeda K, Tanaka H, Kobayashi N, Tanoi K, Yamakawa T, Mori S
Discovery of radioactive silver (110mAg) in spiders and other fauna in the terrestrial environment after the meltdown of Fukushima Dai-ichi nuclear power plant.
Proc Jpn Acad, Ser B, Phys Biol Sci 91: 160-174.

Nozoye T, Nagasaka S, Kobayashi T, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK
The phytosiderophore efflux transporter TOM2 is involved in metal transport in rice.
J Biol Chem 290: 27688–27699.

Nozoye T, Nakanishi H, Nishizawa NK
Transcriptomic analyses of maize ys1 and ys3 mutants reveal maize iron homeostasis. Genom Data 5: 97–99.

2014

Bashir K, Hanada K, Shimizu M, Seki M, Nakanishi H, Nishizawa NK
Transcriptomic analysis of rice in response to iron deficiency and excess.
Rice 7: 18.

Kobayashi T, Itai RN, Nishizawa NK
Iron deficiency responses in rice roots.
Rice 7: 27.

Nakanishi H, Tanaka H, Takeda K, Tanoi K, Hirose A, Nagasaka S, Yamakawa T, Mori S
Radioactive cesium distribution in bamboo (Phyllostachy reticulate (Rupr) K. Koch) shoots after the TEPCO Fukushima Daiichi Nuclear Power Plant disaster.
Soil Sci Plant Nutr 60: 801-808.

Nozoye T, Kim S, Kakei Y, Takahashi M, Nakanishi H, Nishizawa NK
Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean.
Biosci Biotechnol Biochem 78: 1677-1684.

Nozoye T, Tsunoda K, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK
Rice nicotianamine synthase localizes to particular vesicles for proper function.
Plant Signal Behav 9: e28660.

Ogo Y, Kakei Y, Itai RN, Kobayashi T, Nakanishi H, Nishizawa NK
Tissue-specific transcriptional profiling of iron-deficient and cadmium-stressed rice using laser capture microdissection.
Plant Signal Behav 9: e29427.

Ogo Y, Kakei Y, Itai RN, Kobayashi T, Nakanishi H, Takahashi H, Nakazono M, Nishizawa NK
Spatial transcriptomes of iron-deficient and cadmium-stressed rice.
New Phytol 201: 781-794.

Takahashi R, Ishimaru Y, Shimo H, Bashir K, Senoura T, Sugimoto K, Ono K, Suzui N, Kawachi N, Ishii S, Yin YG, Fujimaki S,Yano M, Nishizawa NK, Nakanishi H
From laboratory to field: OsNRAMP5-knockdown rice is a promising candidate for Cd phytoremediation inpaddy fields.
PLoS One 9: e98816.

Xiong H, Guo X, Kobayashi T, Kakei Y, Nakanishi H, Nozoye T, Zhang L, Shen H, Qiu W, Nishizawa NK, Zuo Y
Expression of peanut Iron Regulated Transporter 1 in tobacco and rice plants confers improved iron nutrition.
Plant Physiol Biochem 80: 83-89.

Zhang L, Itai RN, Yamakawa T, Nakanishi H, Nishizawa NK, Kobayashi T
The Bowman-Birk trypsin inhibitor IBP1 interacts with and prevents degradation of IDEF1 in rice.
Plant Mol Biol Rep 32: 841-851.

2013

Aung MS, Masuda H, Kobayashi T, Nakanishi H, Yamakawa T, Nishizawa NK
Iron biofortification of Myanmar rice.
Front Plant Sci 4: 158.

Bashir K, Nozoye T, Ishimaru Y, Nakanishi H, Nishizawa NK
Exploiting new tools for iron bio-fortification of rice.
Biotechnol Adv 31: 1624–1633.

Bashir K, Takahashi R, Akhtar S, Ishimaru Y, Nakanishi H, Nishizawa NK,
The knockdown of OsVIT2 and MIT affects iron localization in rice seed.
Rice 6: 31.

Bashir K, Takahashi R, Nakanishi H, Nishizawa NK
The road to micronutrient biofortification of rice: progress and prospects.
Front Plant Sci 4: 15.

Itai RN, Ogo Y, Kobayashi T, Nakanishi H, Nishizawa NK
Rice genes involved in phytosiderophore biosynthesis are synchronously regulated during the early stages of iron deficiency in roots.
Rice 6: 16.

Kakei Y, Ogo Y, Itai RN, Kobayashi T, Yamakawa T, Nakanishi H, Nishizawa NK
Development of a novel prediction method of cis-elements to hypothesize collaborative functions of cis-element pairs in iron-deficient rice.
Rice 6: 22.

Kobayashi T, Nagasaka S, Senoura T, Itai RN, Nakanishi H, Nishizawa NK
Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation.
Nat Commun 4: 2792.

Masuda H, Aung MS, Nishizawa NK
Iron biofortification of rice using different transgenic approaches.
Rice 6: 40.

Masuda H, Kobayashi T, Ishimaru Y, Takahashi M, Aung MS, Nakanishi H, Mori S, Nishizawa NK
Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene.
Front Plant Sci 4: 132.

Nozoye T, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice.
Plant J 77: 246–260.

Nozoye T, Nakanishi H, Nishizawa NK
Characterizing the crucial components of iron homeostasis in the maize mutants ys1 and ys3.
PLoS One 8: e62567

Takahashi R, Ishimaru Y, Shimo H, Bashir K, Senoura T, Sugimoto K, Ono K, Suzui N, Kawachi N, Ishii S, Yin YG, Fujimaki S, Yano M, Nishizawa NK, Nakanishi H
From laboratory to field: OsNRAMP5-knockdown rice is a promising candidate for Cd phytoremediation in paddy fields.
PLoS One 9: e98816.

Xiong H, Kakei Y, Kobayashi T, Guo X, Nakazono M, Takahashi H, Nakanishi H, Shen H, Zhang F, Nishizawa NK, Zuo Y
Molecular evidence for phytosiderophore-induced improvement of iron nutrition of peanut intercropped with maize in calcareous soil.
Plant Cell Environ 36: 1888-1902.

2012

Bashir K, Ishimaru Y, Shimo H, Kakei Y, Senoura T, Takahashi R, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK
Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron.
Soil Sci Plant Nutr 57: 803-812.

Ishikawa S, Ishimaru Y, Igura M, Kuramata M, Abe T, Senoura T, Hase Y, Arao T, Nishizawa NK, Nakanishi H
Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low cadmium rice.
Proc Natl Acad Sci USA 109: 19166-19171.

Ishimaru Y, Bashir K, Nakanishi H, Nishizawa NK
OsNRAMP5, a major player for constitutive iron and manganese uptake in rice.
Plant Signal Behav 7: 763-766.

Ishimaru Y, Takahashi R, Bashir K, Shimo H, Senoura T, Sugimoto K, Ono K, Yano M, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK
Characterizing the role of rice NRAMP5 in manganese, iron and cadmium transport.
Sci Rep 2: 8.

Kakei Y, Ishimaru Y, Kobayashi T, Yamakawa T, Nakanishi H, Nishizawa NK
OsYSL16 plays a role in the allocation of iron.
Plant Mol Biol 79: 583-594.

Kobayashi T, Itai RN, Aung MS, Senoura T, Nakanishi H, Nishizawa NK
The rice transcription factor IDEF1 directly binds to iron and other divalent metals for sensing cellular iron status.
Plant J 69: 81-91.

Masuda H, Ishimaru H, Aung MS, Kobayashi T, Kakei T, Takahashi M, Higuchi K, Nakanishi H, Nishizawa NK
Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Sci Rep 2: 543.

Mori S, Hirato A, Tanoi K, Takeda K, Yamakawa T, Nakanishi H
Radioactive cesium flow in Rhus vernicifera.
Soil Sci Plant Nutr 58: 611-617.

Suzuki M, Bashir K, Inoue H, Takahashi M, Nakanishi H, Nishizawa NK
Accumulation of starch in Zn-deficient rice.
Rice 5: 9.

Takahashi R, Ishimaru Y, Shimo H, Ogo Y, Senoura T, Nishizawa NK, Nakanishi H
The OsHMA2 transporter is involved in root-to-shoot translocation of Zn and Cd in rice. Plant Cell Environ 35: 1948-1957.

Xiong H, Kobayashi T, Kakei Y, Senoura T, Nakazono M, Takahashi H, Nakanishi H, Shen H, Duan P, Guo X, Nishizawa NK, Zuo Y
AhNRAMP1 iron transporter is involved in iron acquisition in peanut.
J Exp Bot 63: 4437-4446.

2011

Bashir K, Ishimaru Y, ShimoH, Nagasaka S, Fujimoto M, Takanashi H, Tsutsumi N, An G, Nakanishi H, Nishizawa NK
The rice mitochondrial iron transporter is essential for plant growth.
Nat Commun 2: 322.

Bashir K, Ishimaru Y, Nishizawa NK
Identification and characterization of the major mitochondrial Fe transporter in rice.
Plant Signal Behav 6: 1591–1593.

Bashir K, Ishimaru Y, Shimo H, Kakei Y, Senoura T, Takahashi R, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK
Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron.
Soil Sci Plant Nutr 57: 803–812.

Ishimaru Y, Bashir K, Nakanishi H, Nishizawa NK
The role of rice phenolics efflux transporter in solubilizing apoplasmic iron.
Plant Signal Behav 6: 1624–1626.

Ishimaru Y, Bashir K, Nishizawa NK
Zn uptake and translocation in rice plants.
Rice 4: 21–27.

Ishimaru Y, Kakei Y, Shimo H, Bashir K, Sato Y, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK
A rce phenolic efflux transporter is essential for solubilizing precipitated apoplasmic iron in the plant stele.
J Biol Chem 286: 24649–24655.

Lee S, Persson DP, Hansen TH, Husted S, Schjoerring JK, Kim Y-S, Jeon US, Kim Y-K, Kakei Y, Masuda H, Nishizawa NK, An G
Bio-available zinc in rice seeds is increased by activation tagging of nicotianamine synthase.
Plant Biotechnol J 9: 865–873.

Nozoye T, Nagasaka S, Kobayashi T, Takahashi M, Sato Y, Sato Y, Uozumi N, Nakanishi H, Nishizawa NK
Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants.
J Biol Chem 286: 5446-5454.

Ogo Y, Itai RN, Kobayashi T, Aung MS, Nakanishi H, Nishizawa NK
OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.
Plant Mol Biol 75: 593-605.

Rajhi I, Yamauchi T, Takahashi H, Nishiuchi S, Shiono K, Watanabe R, Mliki A, Nagamura Y, Tsutsumi N, Nishizawa NK, Nakazono M
Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses.
New Phytol 190: 351-368.

Shimo H, Ishimaru Y, An G, Yamakawa T, Nakanishi H, Nishizawa NK
Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice.
J Exp Bot 62: 5727–5734.

Takahashi R, Ishimaru Y, Nakanishi H, Nishizawa NK
Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice.
Plant Signal Behav 6: 1813–1816.

Takahashi R, Ishimaru Y, Senoura T, Shimo H, Ishikawa S, Arao T, Nakanishi H, Nishizawa NK
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice.
J Exp Bot 62: 4843–4850.

Takahashi H, Saika H, Matsumura H, Nagamura Y, Tsutsumi N, Nishizawa NK, Nakazono M
Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence.
Ann Bot 108: 253-261.

Zhang M, Senoura T, Yang X, Chao Y, Nishizawa NK
Lhcb2 gene expression analysis in two ecotypes of Sedum alfredii subjected to Zn/Cd treatments with functional analysis of SaLhcb2 isolated from a Zn/Cd hyperaccumulator.
Biotechnol Lett 33: 1865–1871.

Zhang M, Senoura T, Yang X, Nishizawa NK
Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non-hyperaccumulating ecotype of Sedum alfredii Hance.
FEBS Lett 585: 2604–2609.

2010

Bashir K, Ishimaru Y, Nishizawa NK
Iron uptake and loading into rice grains.
Rice 3: 122–130.

Ishimaru Y, Masuda H, Bashir K, Inoue H, Tsukamoto T, Takahashi M, Nakanishi H, Aoki N, Hirose T, Ohsugi R, Nishizawa NK
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.
Plant J 62: 379–390.

Kinoshita N, Berr A, Belin C, Chappuis R, Nishizawa NK, Lopez-Molina L
Identification of growth insensitive to ABA3 (gia3), a recessive mutation affecting ABA signaling for the control of early post-germination growth in Arabidopsis thaliana.
Plant Cell Physiol 51: 239–251.

Kobayashi T, Nakanishi H, Nishizawa NK
Recent insights into iron homeostasis and their application in graminaceous crops.
Proc Jpn Acad, Ser B, Phys Biol Sci 86: 900–913.

Kobayashi T, Nakanishi H, Nishizawa NK
Dual regulation of iron deficiency response mediated by the transcription factor IDEF1. Plant Signal Behav 5: 157–159.

Kobayashi T, Ogo Y, Aung MS, Nozoye T, Itai RN, Nakanishi H, Yamakawa T, Nishizawa NK
The spatial expression and regulation of transcription factors IDEF1 and IDEF2.
Ann Bot 105: 1109–1117.

Takahashi H, Kamakura H, Sato Y, Shiono K, Abiko T, Tsutsumi N, Nagamura Y, Nishizawa NK, Nakazono M
A method for obtaining high quality RNA from paraffin sections of plant tissues by laser microdissection.
J Plant Res 123: 807–813.

2009

Aoyama T, Kobayashi T, Takahashi M, Nagasaka S, Usuda K, Kakei Y, Ishimaru Y, Nakanishi H, Mori S, Nishizawa NK
OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.
Plant Mol Biol 70: 681–692.

Inoue H, Kobayashi T, Nozoye T, TAKAHASHI M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S, Nishizawa NK
Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings.
J Biol Chem 284: 3470–3479.

Ishimaru T, Horigane AK, Ida M, Iwasawa N, San-oh YA, Nakazono M, Nishizawa NK, Masumura T, Kondo M, Yoshida M
Formation of grain chalkiness and changes in water distribution in developing rice caryopses grown under high-temperature stress.
J Cereal Sci 50: 166–174.

Ishimaru Y, Bashir K, Fujimoto M, An G, Itai RN, Tsutsumi N, Nakanishi H, Nishizawa NK
Rice-specific mitochondrial iron-regulated gene (MIR) plays an important role in iron homeostasis.
Mol Plant 2: 1059–1066.

Ito S, Inoue H, Kobayashi T, Yoshiba M, Mori S, Nishizawa NK, Higuchi K
Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco.
Soil Sci Plant Nutr 55: 277–282.

Kakei Y, Yamaguchi I, Kobayashi T, Takahashi M, Nakanishi H, Yamakawa T, Nishizawa NK
A highly sensitive, quick and simple quantification method for nicotianamine and 2′-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants.
Plant Cell Physiol 50: 1988–1993.

Kobayashi T, Itai RN, Ogo Y, Kakei Y, Nakanishi H, Takahashi M, Nishizawa NK
The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes.
Plant J 60: 948–961.

Kwan S, Hamada K, Matsuyama A, Yasuda M, Nakashita H, Yamakawa T
Biotic and abiotic stresses induce AbSAMT1, encoding S-adenosyl-L-methionine salicylic acid carboxyl methyltransferase, in Atropa belladonna.
Plant Biotechnol 25: 207-215.

Lee S, Jeon US, Lee SJ, Kim Y-K, Persson DP, Husted S, Schjørring JK, Kakei Y, Masuda H, Nishizawa NK, An G
Iron fortification of rice seeds through activation of the nicotianamine synthase gene.
Proc Natl Acad Sci USA 106: 22014–22019.

Masuda H, Usuda K, Kobayashi T, Ishimaru Y, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Mori S, Nishizawa NK
Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains.
Rice 2: 155–166.

Matsumoto Y, Suzuki S, Nozoye T, Yamakawa T, Takashima Y, Arakawa T, Tsuji N, Takaiwa F, Hayashi Y
Oral immunogenicity and protective efficiency in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit.
Transgenic Res 18: 1047-1053.

Nozoye T, Takaiwa F, Tsuji N, Yamakawa T, Arakawa T, Hayashi Y, Matsumoto Y
Production of Ascaris suum As14 protein and its fusion protein with cholera toxin B subunit in rice seeds.
J Vet Med Sci 71: 995-1000.

Ogawa I, Nakanishi H, Mori S, Nishizawa NK
Time course analysis of gene regulation under cadmium stress in rice.
Plant Soil 325: 97–108.

Osaki Y, Shirabe T, Nakanishi H, Wakagi T, Yoshimura
Characterization of phytochelatin synthase produced by the primitive red alga Cyanidioschyzon merolae.
Metallomics 1: 353–358.

Sawada  K, Taki A, Yamakawa T, Seki M
Key role for transketolase activity in erythritol production by Trichosporonoides megachiliensis SN-G42.
J Biosci Bioenineer 108: 385-390.

Takahashi M, Nozoye T, Kitajima N, Fukuda N, Hokura A, Terada Y, Nakai I, Ishimaru Y, Kobayashi T, Nakanishi H, Nishizawa NK
In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu.
Plant Soil 325: 39–51.

Tsukamoto T, NAKANISHI H, Uchida H, Watanabe S, Matsuhashi S, Mori S, Nishizawa NK
52Fe translocation in barley as monitored by a Positron-Emitting Tracer Imaging System (PETIS): Evidence for the direct translocation of Fe from roots to young leaves via phloem.
Plant Cell Physiol 50: 48–57.

Usuda K, Wada Y, Ishimaru Y, Kobayashi T, Takahashi M, Nakanishi H, Nagato Y, Mori S, Nishizawa NK
Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect.
Plant Biotechnol J 7: 87–95.

Watanabe S, Ishioka NS, Osa A, Koizumi M, Sekine T, Kiyomiya S, Nakanishi H, Mori S
Production of positron emitters of metallic elements to study plant uptake and distribution.
Radiochim Acta 89: 853.

2008

Inoue H, Takahashi M, Kobayashi T, Suzuki M, Nakanishi H, Mori S, Nishizawa NK
Identification and localisation of the rice nicotianamine aminotransferase gene OsNAAT1 expression suggests the site of phytosiderophore synthesis in rice.
Plant Mol Biol 66: 193-203.

Ishimaru Y, Suzuki M, Ogo Y, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants.
Soil Sci Plant Nutr 54: 417-423.

Kobayashi T, Nakanishi H, Takahashi M, Mori S, Nishizawa NK
Generation and field trials of transgenic rice tolerant to Iron Deficiency.
Rice 1: 144-153.

Masuda H, Suzuki M, Morikawa KC, Kobayashi T, Nakanishi H, Takahashi M, Saigusa M, Mori S, Nishizawa NK
Increase in iron and zinc concentrations in rice grains via the introduction of barley genes involved in phytosiderophore synthesis.
Rice 1: 100-108.

Morikawa CK, Saigusa M, Nishizawa NK, Mori S
Importance of contact between rice roots and co-situs applied fertilizer granules on iron absorption by paddy rice in a calcareous paddy soil.
Soil Sci Plant Nutr 54: 467–472.

Nagasaka S, Takahashi M, Nakanishi-Itai R, Bashir K, Nakanishi H, Mori S, Nishizawa NK
Time course analysis of gene expression over 24 hours in Fe-deficient barley roots.
Plant Mol Biol 69: 621–631.

Ogo Y, Kobayashi T, Nakanishi Itai R, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK
A novel transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants.
J Biol Chem 283: 13407-13417.

Suzuki M, Morikawa CK, Nakanishi H, Takahashi M, Saigusa M, Mori S, Nishizawa NK
Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil.
Soil Sci Plant Nutr 54: 77-85.

Suzuki M, Tsukamoto T, Inoue H, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants.
Plant Mol Biol 66: 609-617.

2007

Bashir K, Nagasaka S, Itai RN, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Expression and enzyme activity of glutathione reductase is upregulated by Fe-deficiency in graminaceous plants.
Plant Mol Biol 65: 277–284.

Chen SC, Liu HW, Lee KT, Yamakawa T
High-efficiency Agrobacterium rhizogenes-mediated transformation of heat inducible sHSP18.2-GUS in Nicotiana tabacum.
Plant Cell Rep 26: 29-37.

Ishimaru Y, Kim S, Tsukamoto T, Oki H, Kobayashi T, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil.
Proc Natl Acad Sci USA 104: 7373-7378.

Ishimaru Y, Masuda H, Suzuki M, Bashir K, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants.
J Exp Bot 58: 2909-2915.

Ito S, Inoue H, Kobayashi T, Yoshiba M, Mori S, Nishizawa N, Higuchi K
Interspecies compatibility of NAS1 gene promoters.
Plant Physiol Biochem 45: 270–276.

Kobayashi T, Ogo Y, Itai RN, Nakanishi H, Takahashi M, Mori S, Nishizawa NK
The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants.
Proc Natl Acad Sci USA 104: 19150-19155.

Kobayashi T, Yoshihara T, Itai RN, Nakanishi H, Takahashi M, Mori S, Nishizawa NK
Promoter analysis of iron-deficiency-inducible barley IDS3 gene in Arabidopsis and tobacco plants.
Plant Physiol Biochem 45: 262–269.

Lee KT, Chen SC, Chiang BL, Yamakawa T
Heat-inducible production of beta-glucuronidase in tobacco hairy root cultures.
Appl Microbiol Biotechnol 73:1047-53.

Nozoye T, Inoue H, Takahashi M, Ishimaru Y, Nakanishi H, , Mori S, Nishizawa NK
The expression of iron homeostasis-related genes during rice germination.
Plant Mol Biol 64: 35-47.

Ogo Y, Nakanishi Itai R, Nakanishi H, Kobayashi T, Takahashi M, Mori S, Nishizawa NK
The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions.
Plant J 51: 366-377.

Suzuki T, Tamura S, Nakannsihi H, Tashiro M, Nishizawa NK, Yoshimura E
Reduction of aluminum toxicity by 2-isopropylmalic acid in the budding yeast Saccharomyces cerevisiae.
Biol Trace Elem Res
120: 257-263.

Wada Y, Yamaguchi I, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.
Biosci Biotechnol Biochem 71: 435-441.

2006

Bashir K, Inoue H, Nagasaka S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants.
J Biol Chem 281: 32395-32402.

Bashir K, Nishizawa NK
Deoxymugineic acid synthase.
Plant Signal Behav 1: 290–292.

Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.
Plant J 45: 335-346.

Ko S, Liu JR, Yamakawa T, Matsumoto Y
Expression of the protective antigen (SpaA) in transgenic hairy roots of tobacco.
Plant Mol Biol Rep 24: 251.

Nakada M, Komatsu M, Ochiai T, Ohtsu K, Nakazono M, Nishizawa NK, Nitta K, Nishiyama R, Kameya T, Kanno A
Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection.
Plant Sci 170: 143-150.

Nakanishi H, Ogawa I, Ishimaru Y, Mori S, Nishizawa NK
Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice.
Soil Sci Plant Nutr 52: 464-469.

Ogo Y, Nakanishi Itai, R, Nakanishi H, Inoue H, Kobayashi T, Suzuki M, Takahashi M, Mori S, Nishizawa NK.
Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants.
J Exp Bot 57: 2867-2878.

Suganda AG, Sukrasno, Nishiyama Y, Yamakawa T, Sugiyama N
Random amplified polymorphic DNA analysis to distinguish Brugmansia suaveolens, B. candida and B. versicolor.
Plant Biotechnol 23: 519-520.

Suzuki M, Takahashi M, Tsukamoto T, Watanabe S, Matsuhashi S, Yazaki J, Kishimoto N, Kikuchi S, Nakanishi H, Mori S, Nishizawa NK.
Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley.
Plant J 48: 85-97.

Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa NK, Gomi K, Shimada A, Kitano H, Ashikari M, Matsuoka M.
GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers.
Plant J 47: 427-444.

Tsukamoto T, Nakanishi H, Kiyomiya S, Watanabe S, Matsuhashi S, Nishizawa NK, Mori S.
52
Mn translocation in barley as monitored using a positron-emitting tracer imaging system (PETIS).
Soil Sci Plant Nutr 52: 717-725.

Wada Y, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK.
Metabolic engineering of Saccharomyces cerevisiae producing nicotianamine: Potential for industrial biosynthesis of a novel antihypertensive substrate.
Biosci Biotechnol Biochem 70: 1408-1415.

2005

Blamey FPC, Nishizawa NK, Yoshimura E
Digital microscopy: A useful technique for measuring root elongation in solution.
Soil Sci Plant Nutr 51: 705–708.

Ishimaru, Y, Suzuki M, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
OsZIP4, a novel zinc-regulated zinc transporter in rice.
J Exp Bot 55: 3207-3214.

Kawagoe Y, Suzuki K, Tasaki M, Yasuda H, Akagi K, Katoh E, Nishizawa NK, Ogawa M, Takaiwa F
The critical role of disulfide bond formation in protein sorting in the endosperm of rice.
Plant Cell 17: 1141-1153.

Kim S, Takahashi T, Higuchi K, Tsunoda K, Nakanishi H, Yoshimura E, Mori S, Nishizawa NK.
Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants.
Plant Cell Physiol 46:1809-1818.

Kobayashi T, Suzuki M, Inoue H, Itai RN, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements.
J Exp Bot 56: 1305–1316.

Takagi H, Saito S, Yang L, Nagasaka S, Nishizawa NK, Takaiwa F
Oral immunotherapy against a pollen allergy using a seed-based peptide vaccine.
Plant Biotechnol J 3: 521-533.

2004

Blarney FPC, Nishizawa NK, Yoshimura E
Timing, magnitude, and location of initial soluble aluminum injuries to Mungbean roots.
Soil Sci Plant Nutr 50: 67–76.

Inoue H, Mizuno D, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport.
Soil Sci Plant Nutr 50: 1133–1140.

Kobayashi T, Nakayama Y, Takahashi M, Inoue H, Nakanishi H, Yoshihara T, Mori S, Nishizawa NK
Construction of artificial promoters highly responsive to iron deficiency.
Soil Sci Plant Nutr 50: 1167–1175.

Koike S, Inoue H, Mizuno D, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem.
Plant J 39: 415–424.

Morikawa CK, Saigusa M, Nakanishi H, Nishizawa NK, Hasegawa K, Mori S
Co-situs application of controlled-release fertilizers to alleviate iron chlorosis of paddy rice grown in calcareous soil.
Soil Sci Plant Nutr 50: 1013–1021.

Nagasaka S, Nishizawa NK, Mori S, Yoshimura E
Metal metabolism in the red alga Cyanidium caldarium and its relationship to metal tolerance.
Biometals 17: 177–181.

Nishiyama Y, Yamakawa T
Effect of medium composition on the production of anthocyanins by hairy root cultures of Ipomoea batatas.
Plant Biotechnol 21: 411-414.

Nozoye T, Itai RN, Nagasaka S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Diurnal changes in the expression of genes that participate im phytosiderophore synthesis in rice.
Soil Sci Plant Nutr 50: 1125–1131.

Oki H, Kim S, Nakanishi H, Takahashi M, Yamaguchi H, Mori S, Nishizawa NK
Directed evolution of yeast ferric reductase to produce plants with tolerance to iron deficiency in alkaline soils.
Soil Sci Plant Nutr 50: 1159–1165.

Singh K, Sharma HC, Singh CS, Singh Y, Nishizawa NK, Mori S
Effect of polyolefin resin coated slow release iron fertilizer and its methods of application on rice production in calcareous soil.
Soil Sci Plant Nutr 50: 1037–1042.

Terauchi K, Asakura T, Nishizawa NK, Matsumoto I, Abe K
Characterization of the genes for two soybean aspartic proteinases and analysis of their different tissue-dependent expression.
Planta 218: 947–957.

Tsukamoto T, Uchida H, Nakanishi H, Nishiyama S, Tsukada H, Matsuhashi S, Nishizawa NK, Mori S
H215O translocation in rice was enhanced by 10μm 5-aminolevulinic acid as monitored by positron emitting tracer imaging system (PETIS).
Soil Sci Plant Nutr 50: 1085–1088.

2003

Chiba A, Ishida H, Nishizawa NK, Makino A, Mae T
Exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts by specific bodies in naturally senescing leaves of wheat.
Plant Cell Physiol 44: 914–921.

Hirano H, Shimomura K, Yamakawa T
Responses of transformed roots of Atropa belladonna to treatment with salicylic acid and other phenolic compounds.
Plant Biotechnol 20: 97-100.

Inoue H, Higuchi K, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and diferentially regulated by iron.
Plant J 36: 366–381.

Kobayashi T, Nakayama Y, Itai RN, Nakanishi H, Yoshihara T, Mori S, Nishizawa NK
Identification of novel cis-acting elements, IDE1 and IDE2, of the barley IDS2 gene promoter conferring iron-deficiency-inducible, root-specific expression in heterogeneous tobacco plants.
Plant J 36: 780–793.

Kobayashi T, Yoshihara T, Jiang T, Goto F, Nakanishi H, Mori S, Nishizawa NK
Combined deficiency of iron and other divalent cations mitigates the symptoms of iron deficiency in tobacco plants.
Physiol Plant 119: 400–408.

Mizuno D, Higuchi K, Sakamoto T, Nakanishi H, Mori S, Nishizawa NK
Three nicotianamine synthase genes isolated from maize are differentially regulated by iron nutritional status.
Plant Physiol 132: 1989–1997.

Nagasaka S, Nishizawa NK, Watanabe T, Mori S, Yoshimura E
Evidence that electron-dense bodies in Cyanidium caldarium have an iron-storage role.
Biometals 16: 465–470.

Takahashi M, Terada Y, Nakai I, Nakanishi H, Yoshimura E, Mori S, Nishizawa NK
Role of nicotianamine in the intracellular delivery of metals and plant reproductive development.
Plant Cell 15: 1263–1280.

Yoshihara T, Masuda T, Jiang T, Goto F, Mori S, Nishizawa NK
Analysis of some divalent metal contents in tobacco expressing the exogenous soybean ferritin gene.
J Plant Nutr 26: 2253–2265.

2002

Bughio N, Yamaguchi H, Nishizawa NK, Nakanishi H, Mori S
Cloning an iron‐regulated metal transporter from rice.
J Exp Bot 53: 1677–1682.

Fukami H, Asakura T, Hirano H, Abe K, Shimomura K, Yamakawa T
Salicylic acid carboxyl methyltransferase induced in hairy root culture of Atropa belladonna after treatment with exogenously added salicylic acid.
Plant Cell Physiol 43: 1054-1058.

Nagasaka S, Nishizawa NK, Negishi T, Satake K, Mori S, Yoshimura E
Novel iron-storage particles may play a role in aluminum tolerance of Cyanidium caldarium.
Planta 215: 399–404.

Nakanishi H, Kiyomiya S, Tsukamoto T, Tsukada H, Uchida H, Mori S
Water (H215O) flow in rice is regulated by the concentration of nutrients as monitored by positron multi-probe system (PMPS).
Soil Sci Plant Nutr 48: 759–762.

Negishi T, Nakanishi H, Yazaki J, Kishimoto N, Fujii F, Shimbo K, Yamamoto K, Sakata K, Sasaki T, Kikuchi S, Mori S, Nishizawa NK
cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots.
Plant J 30: 83–94.

Sudo H, Yamakawa T, Yamazaki M, Aimi N, Saito K
Bioreacter production of camptothecin by hairy root cultures of Ophiorrhiza pumila.
Biotechnol Lett 24: 359-363.

Yamaguchi H, Nishizawa N-K, Nakanishi H, Mori S
IDI7, a new iron‐regulated ABC transporter from barley roots, localizes to the tonoplast.
J Exp Bot 53: 727–735.

2001

Bughio N, Nakanishi H, Kiyomiya S, Matsuhashi S, Ishioka N-S, Watanabe S, Uchida H, Tsuji A, Osa A, Kume T, Hashimoto S, Sekine T, Mori S
Real-time [11C]methionine translocation in barley in relation to mugineic acid phytosiderophore biosynthesis.
Planta 213: 708–715.

Hayashi Y, Yamada K, Shimada T, Matsushima R, Nishizawa N, Nishimura M, Hara-Nishimura I
A proteinase-storing body that prepares for cell death or stresses in the epidermal cells of Arabidopsis.
Plant Cell Physiol 42: 894–899.

Higuchi K, Takahashi M, Nakanishi H, Kawasaki S, Nishizawa NK, Mori S
Analysis of transgenic rice containing barley nicotianamine synthase gene.
Soil Sci Plant Nutr 47: 315–322.

Higuchi K, Tani M, Nakanishi H, Yoshiwara T, Goto F, Nishizawa NK, Mori S
The expression of a barley HνNAS1 nicotianamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in roots.
Biosci Biotechnol Biochem 65: 1692–1696.

Higuchi K, Watanabe S, Takahashi M, Kawasaki S, Nakanishi H, Nishizawa NK, Mori S
Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions.
Plant J 25: 159–167.

Hisada A, Yoshida T, Kubota S, Nishizawa NK, Furuya M
Technical advance: An automated device for cryofixation of specimens of electron microscopy using liquid helium.
Plant Cell Physiol 42: 885–893.

Kiyomiya S, Nakanishi H, Uchida H, Nishiyama S, Tsukada H, Ishioka NS, Watanabe S, Osa A, Mizuniwa C, Ito T, Matsuhashi S, Hashimoto S, Sekine T, Tsuji A, Mori S
Light activates H215O flow in rice: Detailed monitoring using a positron-emitting tracer imaging system (PETIS).
Physiol Plant 113: 359–367.

Kiyomiya S, Nakanishi H, Uchida H, Tsuji A, Nishiyama S, Futatsubashi M, Tsukada H, Ishioka NS, Watanabe S, Ito T, Mizuniwa C, Osa A, Matsuhashi S, Hashimoto S, Sekine T, Mori S
Real time visualization of 13N-translocation in rice under different environmental conditions using Positron Emitting Tracer Imaging System.
Plant Physiol 125: 1743–1753.

Kobayashi T, Nakanishi H, Takahashi M, Kawasaki S, Nishizawa N-K, Mori S
In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2’-deoxymugineic acid to mugineic acid in transgenic rice.
Planta 212: 864–871.

Kokubo T, Ambe-Ono Y, Nakamura M, Ishida H, Yamakawa T, Kodama T
Promotive effect of auxins on UDP-glucose: flavonol glucosyltransferase activity in Vitis sp. cell cultures.
J Biosci Bioeng 91 :564-569.

Lee KT, Hirano H, Yamakawa T, Kodama T, Igarashi Y, Shimomura K
Response of transformed root culture of Atropa belladonna to salicylic acid stress.
J Biosci Bioeng 91: 586-589.

Nishiyama Y, Yamakawa T
Light-controlled expression of beta-glucuronidase gene driven by palg2b promoter from populus kitakamiensis in Nicotiana plumbaginifolia.
Plant Biotechnol 18: 67-70.

Suzuki K, Nakansihi H, Nishizawa NK, Mori S
Analysis of upstream region of nicotianamine synthase gene from Arabidopsis thaliana: Presence of putative ERE-like sequence.
Biosci Biotechnol Biochem 65: 2794–2797.

Takahashi M, Nakanishi H, Kawasaki S, Nishizawa NK, Mori S
Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes.
Nat Biotech 19: 466–469.

2000

Itai R, Suzuki K, Yamaguchi H, Nakanishi H, Nishizawa N-K, Yoshimura E, Mori S
Induced activity of adenine phosphoribosyltransferase (APRT) in iron‐deficient barley roots: a possible role for phytosiderophore production.
J Exp Bot 51: 1179–1188.

Mori S, Kiyomiya S, Nakanishi H, Ishioka NS, Watanabe S, Osa A, Matsuhashi S, Hashimoto S, Sekine T, Uchida H, Nishiyama S, Tsukada H, Tsuji A
Visualization of 15O-water flow in tomato and rice in the light and dark using a positron-emitting tracer imaging system (PETIS).
Soil Sci Plant Nutr 46: 975–979.

Nakanishi H, Yamaguchi H, Sasakuma T, Nishizawa NK, Mori S
Two dioxygenase genes, Ids3 and Ids2, from Hordeum vulgare are involved in the biosynthesis of mugineic acid family phytosiderophores.
Plant Mol Biol 44: 199–207.

Singh K, Sasakuma T, Bughio N, Takahashi M, Nakanishi H, Yoshimura E, Nishizawa NK, Mori S
Ability of ancestral wheat species to secrete mugineic acid family phytosiderophores in response to iron deficiency.
J Plant Nutr 23: 1973–1981.

Yamaguchi H, Nakanishi H, Nishizawa NK, Mori S
Induction of the IDI1 gene in Fe-deficient barley roots: A gene encoding a putative enzyme that catalyses the methionine salvage pathway for phytosiderophore production.
Soil Sci Plant Nutr 46: 1–9.

Yamaguchi H, Nakanishi H, Nishizawa NK, Mori S
Isolation and characterization of IDI2, a new Fe‐deficiency‐induced cDNA from barley roots, which encodes a protein related to the α subunit of eukaryotic initiation factor 2B (eIF2Bα).
J Exp Bot 51: 2001–2007.

Yoshimura E, Sakaguchi T, Nakanishi H, Nishizawa NK, Nakai I, Mori S
Characterization of the chemical state of iron in the leaves of wild-type tomato and of a nicotianamine-free mutant Chloronerva by X-ray absorption near-edge structure (XANES).
Phytochem Anal 11: 160–162.

Yoshimura E, Sato N, Nishizawa NK, Satake K, Mori S
Accumulation of metals in the cell walls of the liverwort, Scapania undulata.
J Environ Sci Health A 35: 837–847.

1999

Higuchi K, Nakanishi H, Suzuki K, Nishizawa NK, Mori S
Presence of nicotianamine synthase isozymes and their homologues in the root of graminaceous plants.
Soil Sci Plant Nutr 45: 681–691.

Higuchi K, Suzuki K, Nakanishi H, Yamaguchi H, Nishizawa N-K, Mori S
Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores.
Plant Physiol 119: 471–480.

Kaneko M, Yoshimura E, Nishizawa NK, Mori S
Time course study of aluminum-induced callose formation in barley roots as observed by digital microscopy and low-vacuum scanning electron microscopy.
Soil Sci Plant Nutr 45: 701–712.

Lee KT, Suzuki T, Yamakawa T, Kodama T, Igarashi Y, Shimomura K
Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net.
Plant Cell Rep 18: 567-571.

Moriwaki M, Yamakawa T, Washio T, Kodama T, Igarashi Y
A comparispn of GUS activity in liquid- and air-heat shock treatments performed on transgenic Nicotiana plumbaginifolia harboring Arabidopsis HSP18.2 promoter-GUS chimera gene.
Plant Biotechnol 16: 303-305

Moriwaki M, Yamakawa T, Washio T, Kodama T, Igarashi Y
Delayed recovery of beta-glucronidase activity driven by an Arabidopsis heat shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia.
Plant Cell Rep 19: 96-100.

Moriwaki M, Yamakawa T, Washio T, Kodama T, Igarashi Y
Suppressed phenylalanine ammonia-lyase activity after heat shock in transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2-parsley PAL2 chimera gene.
J Ferment Bioeng 87: 588-593.

Nakanishi H, Bughio N, Matsuhashi S, Ishioka N-S, Uchida H, Tsuji A, Osa A, Sekine T, Kume T, Mori S
Visualizing real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a Positron Emitting Tracer Imaging System (PETIS).
J Exp Bot 50: 637–643.

Oki H, Yamaguchi H, Nakanishi H, Mori S
Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco.
Plant Soil 215: 211–220.

Sakaguchi T, Nishizawa NK, Nakanishi H, Yoshimura E, Mori S
The role of potassium in the secretion of mugineic acids family phytosiderophores from iron-deficient barley roots.
Plant Soil 215: 221–227.

Sekiguchi S, Yamakawa T, Kodama T, Smith SM, Yeoman MM
Characterization of chilli pepper hairy roots expressing the parsely PAL2 gene.
Plant Biotechnol 16: 153-158.

Suzuki K, Higuchi K, Nakanishi H, Nishizawa NK, Mori S
Cloning of nicotianamine synthase genes from Arabidopsis thaliana.
Soil Sci Plant Nutr 45: 993–1002.

Takahashi M, Yamaguchi H, Nakanishi H, Shioiri T, Nishizawa N-K, Mori S
Cloning two genes for nicotianamine aminotransferase, a critical enzyme in iron acquisition (Strategy II) in graminaceous plants.
Plant Physiol 121: 947–956.

1998

Ando M, Shimomura K, Yamakawa T, Ishimaru K
Plant regeneration from Wahlenbergia marginata hairy root cultures.
Plant Biotechnol 15: 39-40.

Ishimaru K, Ando M, Yamakawa T, Touno K, Shimomura K
Polyacetylene production in transformed root cultures of Campanula lactiflora.
Narual Medicines 52: 448-451.

Kanazawa K, Higuchi K, Nakanishi H, Kishi-Nishizawa N, Mori S
Characterizing nicotianamine aminotransferase: Improving its assay system and details of the regulation of its activity by Fe nutrition status.
Soil Sci Plant Nutr 44: 717–721.

Lee KT, Yamakawa T, Kodama T, Igarashi Y, Shimomura K
Effects of aeration on tropane alkaloid production by transformed root of Atropa belladonna in flask cultures.
J Ferment Bioeng 86: 614-616.

Lee KT, Yamakawa T, Kodama T, Igarashi Y, Shimomura K
Effects of chemicals on alkaloid production by transformed roots of belladonna.
Phytochemistry 49: 2343-2347.

Suzuki K, Itai R, Suzuki K, Nakanishi H, Nishizawa N-K, Yoshimura E, Mori S
Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots.
Plant Physiol 116: 725–732.

Yamakawa T, Sekiguchi S, Kodama T, Smith SM, Yeoman MM
Transformation of chilli Pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene.
Plant Biotechnol 15: 189-193.

Yoshimura E, Kitai K, Nishizawa NK, Satake K, Mori S, Yamazaki S
Accumulation of metals and cellular distribution of aluminum in the liverwort Scapania undulata, in acidic and neutral streams in Japan.
J Environ Sci Health A 33: 671–680.

1997

Ando M, Shimomura K, Yamakawa T, Ishimaru K
Polyacetylene production in hairy cultures of Wahenbergia marginata.
J Plant Physiol 151: 759-762.

Bughio N, Takahashi M, Yoshimura E, Nishizawa N-K, Mori S
Light-dependent iron transport into isolated barley chloroplasts.
Plant Cell Physiol 38: 101–105.

Ishimaru K, Tasaki S, Tanaka N, Shimomura K, Yamakawa T, Yoshihara K
Polyacetylenes in hairy root cultures of Lobelia siphilitica.
Jap J Food Chem 4: 85-88.

Suzuki K, Kanazawa K, Higuchi K, Nishizawa NK, Mori S
Immunological characterization of a 36 kDa Fe deficiency specific peptide in barley roots.
Biometals 10: 77–84.

Ando M, Shimomura K, Yamakawa T, Ishimaru K
Polyacetylene production in hairy cultures of Wahenbergia marginata.
J Plant Physiol 151: 759-762.

Bughio N, Takahashi M, Yoshimura E, Nishizawa N-K, Mori S
Light-dependent iron transport into isolated barley chloroplasts.
Plant Cell Physiol 38: 101–105.

Ishimaru K, Tasaki S, Tanaka N, Shimomura K, Yamakawa T, Yoshihara K
Polyacetylenes in hairy root cultures of Lobelia siphilitica.
Jap J Food Chem 4: 85-88.

Suzuki K, Kanazawa K, Higuchi K, Nishizawa NK, Mori S
Immunological characterization of a 36 kDa Fe deficiency specific peptide in barley roots.
Biometals 10: 77–84.

1996

Barnes SA, Nishizawa NK, Quaggio RB, Whitelam GC, Chua NH
Far-red light blocks greening of Arabidopsis seedlings via a phytochrome A-mediated change in plastid development.
Plant Cell 8: 601–615.

Higuchi K, Kanazawa K, Nishizawa N-K, Mori S
The role of nicotianamine synthase in response to Fe nutrition status in Gramineae.
Plant Soil 178: 171–177.

Higuchi K, Nishizawa N, Römheld V, Marschner H, Mori S
Absence of nicotianamine synthase activity in the tomato mutant “chloronerva.”
J Plant Nutr 19: 1235–1239.

Miyawaki O, Yoshikawa M, Kuwaharada H, Nakamura K, Yamakawa T, Kodama T, Ito K, Nakajima I
Application of shield hot-wire viscosity sensor to monitoring cultivations of Xanthomonas campestris and Nicotiana tabacum BY-2.
J Ferment Bioeng 82: 68-72.

Sekiguchi S, Yamakawa T, Kodama T, Smith SM, Yeoman MM
Establishment of hairy root culture of chilli Pepper (Capsicum frutescens).
Plant Tissue Culture Letters 13: 219-221.

Takizawa R, Nishizawa N-K, Nakanishi H, Mori S
Effect of iron deficiency on S‐adenosylmethionine synthetase in barley roots.
J Plant Nutr 19: 1189–1200.

1995

Suzuki K, Hirano H, Yamaguchi H, Irifune T, Nishizawa NK, Chino M, Mori S
Partial amino acid sequences of a peptide induced by Fe deficiency in barley roots.
Iron Nutirition in Soils and Plants, 363-369 (J Abadia (ed), Kluwer academic Publicaions).
Nakamura S, Hayashi H, Mori S, Chino M
Detection and characterization of protein kinases in rice phloem sap.
Plant Cell Physiol 36: 19–27.
Kanazawa K, Higuchi K, Nishizawa N-K, Fushiya S, Mori S
Detection of two distinct isozymes of nicotianamine aminotransferase in Fe-deficient barley roots.
J Exp Bot 46: 1241–1244.
Higuchi K, Nishizawa N-K, Yamaguchi H, Römheld V, Marschner H, Mori S
Response of nicotianamine synthase activity to Fe-deficiency in tobacco plants as compared with barley.
J Exp Bot 46: 1061–1063

1994

Okumura N, Nishizawa N-K, Umehara Y, Ohata T, Nakanishi H, Yamaguchi T, Chino M, Mori S (1994) A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare. Plant Mol Biol 25: 705–719
Nishizawa NK, Mori S, Watanabe Y, Hirano H (1994) Ultrastructural Localization of the Basic 7S Globulin in Soybean (Glycine max) Cotyledons. Plant Cell Physiol 35: 1079–1085
Kanazawa K, Higuchi K, Nishizawa N-K, Fushiya S, Chino M, Mori S (1994) Nicotianamine aminotransferase activities are correlated to the phytosiderophore secretions under Fe-deficient conditions in Gramineae. J Exp Bot 45: 1903–1906
Higuchi K, Kanazawa K, Nishizawa N-K, Chino M, Mori S (1994) Purification and characterization of nicotianamine synthase from Fe-deficient barley roots. Plant Soil 165: 173–179

1993

Singh K, Chino M, Nishizawa NK, Ohata T, Mori S (1993) Genotypic variation among Indian graminaceous species with respect to phytosiderophore secretion. In PJ Randall, E Delhaize, RA Richards, R Munns, eds, Genetic Aspects of Plant Mineral Nutrition. Springer Netherlands, pp 335–339
Ohata T, Kanazawa K, Mihashi S, Kishi-Nishizawa N, Fushiya S, Nozoe S, Chino M, Mori S (1993) Biosynthetic pathway of phytosiderophores in iron-deficient graminaceous plants. Soil Science and Plant Nutrition 39: 745–749
Nishizawa Y, Kishimoto N, Saito A, Hibi T (1993) Sequence variation, differential expression and chromosomal location of rice chitinase genes. Molecular Genetics and Genomics
Jurkevitch E, Hadar Y, Chen Y, Chino M, Mori S (1993) Indirect utilization of the phytosiderophore mugineic acid as an iron source to rhizosphere fluorescent Pseudomonas. Biometals 6: 119–123
Kanazawa K, Mihashi S, Nishizawa NK, Chino M, Mori S (1993) Biosynthetic pathway of phytosiderophores in iron-deficient graminaceous plants: A new assay system for the detection of nicotianamine amino-transferase activity. Plant Soil 155-156: 103–105
Mori S, Okumura N, Nakanishi H, Umehara Y, Chino M, Nishizawa NK (1993) Expression of a gene (Ids2) induced by Fe deficiency treatment in the roots of Hordeum vulgare L. Plant Soil 155-156: 135–138
Nagatani A, Nishizawa NK, Mori S, Kay SA, Chua N-H, Furuya M (1993) Light Regulation of Hypocotyl Elongation and Greening in Transgenic Tobacco Seedlings That Over-Express Rice Phytochrome A. Plant Cell Physiol 34: 825–833
Nakamura S, Hayashi H, Mori S, Chino M (1993) Protein Phosphorylation in the Sieve Tubes of Rice Plants. Plant Cell Physiol 34: 927–933
Nakanishi H, Okumura N, Umehara Y, Nishizawa N-K, Chino M, Mori S (1993) Expression of a Gene Specific for Iron Deficiency (Ids3) in the Roots of Hordeum vulgare. Plant Cell Physiol 34: 401–410

1992

Chongpraditnun P, Mori S, Chino M (1992) Excess Copper Induces A Cytosolic Cu,Zn-Superoxide Dismutase in Soybean Root. Plant Cell Physiol 33: 239–244
Mihashi S, Sikora LJ, Buyer JS, Fushiya S, Mori S (1992) Development and Characterization of a Monoclonal Antibody to Phytosiderophores. Plant Cell Physiol 33: 151–156
Okumura N, Nishizawa N-K, Umehara Y, Ohata T, Mori S (1992) Iron deficiency specific cDNA (Ids1) with two homologous cysteine rich mt domains from the roots of barley. Journal of Plant Nutrition 15: 2157–2172

1991

Mihashi S, Mori S, Nishizawa N (1991) Enhancement of ferric-mugineic acid uptake by iron deficient barley roots in the presence of excess free mugineic acid in the medium. Plant Soil 130: 135–141
Mori S, Nishizawa N, Hayashi H, Chino M, Yoshimura E, Ishihara J (1991) Why are young rice plants highly susceptible to iron deficiency? Plant Soil 130: 143–156
Okumura N, Nishizawa N-K, Umehara Y, Mori S (1991) An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains. Plant Mol Biol 17: 531–533
Shojima S, Nishizawa N-K, Fushiya S, Nozoe S, Takagi S, Mori S, Chino M (1991) Cross-reactivities of anti-nicotianamine antibody with mugineic acid and related compounds. Soil Science and Plant Nutrition 37: 159–162

1990

Mori S, Kishi-Nishizawa N, Fujugaki J (1990) Identification of rye chromosome 5R as a carrier of the genes for mugineic acid synthetase and 3-hydroxymugineic acid synthetase using wheat-rye addition lines. 遺伝学雑誌 65: 343–352
Shojima S, Nishizawa N-K, Fushiya S, Nozoe S, Irifune T, Mori S (1990) Biosynthesis of Phytosiderophores In Vitro Biosynthesis of 2′-Deoxymugineic Acid from l-Methionine and Nicotianamine. Plant Physiol 93: 1497–1503

1989

Mihashi S, Mori S (1989) Characterization of mugineic-acid-Fe transporter in Fe-deficient barley roots using the multi-compartment transport box method. Biol Metals 2: 146–154
Mori S, NISHIZAWA N (1989) Identification of Barley Chromosome No. 4, Possible Encoder of Genes of Mugineic acid Synthesis from 2′ -Deoxymugineic acid Using Wheat-Barley Addition Lines. Plant Cell Physiol 30: 1057–1061
Nishizawa N-K, Mori S (1989a) Microbodies containing crystalloid inclusions in rice root cells. Soil Science and Plant Nutrition 35: 485–490
Nishizawa NK, Mori S, Takahashi S, Ueda T (1989) Mugineic acid secretion by cultured barley cells derived from anther. Protoplasma 148: 164–166
Nishizawa N, Mori S (1989b) Ultrastructure of the Thylakoid Membrane in Tomato Leaf Chloroplast Revealed by Liquid Helium Rapid-Freezing and Substitution-Fixation Method. Plant Cell Physiol 30: 1–7
Shojima S, Nishizawa NK, Fushiya S, Nozoe S, Kumashiro T, Nagata T, Ohata T, Mori S (1989a) Biosynthesis of nicotianamine in the suspension-cultured cells of tobacco (Nicotiana megalosiphon). Biol Metals 2: 142–145
Shojima S, Nishizawa N-K, Mori S (1989b) Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine. Plant Cell Physiol 30: 673–677

1988

Mori S, Hachisuka M, Kawai S, Takagi S, Kishi‐Nishizawa N (1988) Peptides related to phytosiderophore secretion by Fe‐deficient barley roots. Journal of Plant Nutrition 11: 653–662

1987

Mori S, Nishizawa N (1987) Methionine as a Dominant Precursor of Phytosiderophores in Graminaceae Plants. Plant Cell Physiol 28: 1081–1092
Mori S, Nishizawa N, Kawai S, Sato Y, Takagi S (1987) Dynamic state of mugineic acid and analogous phytosiderophores in Fe‐deficient barley. Journal of Plant Nutrition 10: 1003–1011
Nishizawa N, Mori S (1987) The particular vesicle appearing in barley root cells and its relation to mugineic acid secretion. Journal of Plant Nutrition 10: 1013–1020
Shojima DS, Nishizawa NK, Mori S (1987) Do intrathylakoidal inclusions really contain RuBPCase? Protoplasma 140: 187–189

1986

Nishizawa N, Mori S (1986) Occurrence of Stress Grains in the Root Cells of Broad Bean and Barley Plants under Desiccation Conditions. Soil Science and Plant Nutrition 32: 599–608

1984

Nishizawa N, Mori S (1984) Desiccation-Induced Heterophagy in Corn Root Cells. J Electron Microsc (Tokyo) 33: 230–235

1981

Nishizawa N, Mori S (1981) A Vesicle Containing Fibrous Materials Appearing in Water-Stressed Corn Root Cells. Plant Cell Physiol 22: 933–938

1980

Nishizawa N, Mori S (1980a) Vacuole formation as a result of intracellular digestion: Acid phosphatase localization as associated with plasmalemma-invagination and vacuole formation. Soil Science and Plant Nutrition 26: 525–540
Nishizawa N, Mori S (1980b) Electronmicroscope-autoradiographical evidence for the incorporation of exogenous protein into rice root cells. Plant Cell Physiol 21: 493–496

1979

Mori S, Nishizawa N (1979) Nitrogen absorption by plant root from the culture medium where organic and inorganic nitrogen coexist. Soil Science and Plant Nutrition 25: 51–58

1978

Nishizawa N, Mori S (1978) Endocytosis (heterophagy) in plant cells: Involvement of ER and ER-derived vesicles. Plant Cell Physiol 19: 717–730

1977

NISHIZAWA N, Mori S (1977) Invagination of plasmalemma: Its role in the absorption of macromolecules in rice roots. Plant Cell Physiol 18: 767–782